access intranet after hours circle-arrow apply blog caret circle arrow close closer look community outreach community outreach contact contact us down arrow facebook lock solid find a provider find a clinical trial find a provider find a researcher find faculty find-a-service how to apply join leadership left arrow locations logo make a gift map location maximize minimize my chart my chart notification hp notification lp next chevron right nxt prev pay your bill play previous quality and safety refer a patient request a speaker request appointment request an appointment residents corner rss search search jobs Asset 65 submit a story idea symptom checker Arrow Circle Up twitter youtube Dino Logo External Link University Logo Color University Logo Solid Health Logo Solid Arrow Right Circle Book Calendar Date Calendar Search Date Diploma Certificate Dollar Circle Donate Envelope Graduation Cap Map Pin Map Search Phone Pills Podcast

Mass Cytometry Resources



Photo of Fluidigm Helios CyTOF machine

Mass cytometry is a single-cell detection technology based on inductively coupled plasma mass spectrometry. Antibodies or other reagents tagged with rare metal isotopes can be used to probe the proteome and potentially over 100 available parameters can be simultaneously measure on a single cell without the issue of spectral overlap typical for classical, fluorochrome-based flow cytometry or tissue/cell autofluorescecne. The in house instrument is a CyTOF2.1 (Helios), capable of measuring 135 different mass channels (75-209amu) at a throughput of 1000 cells/sec.

Illustration of Helios workflow
Figure 3. Mass cytometry workflow. Cells labeled with metal-conjugated antibodies in solution (A) are injected into the nebulizer (B). They are aerosolized and reduced to single cell-containing droplets. The cells are directed to the ICP torch, where they are vaporized, atomized, and ionized in the plasma (C). The high pass optic removes the low-mass ions (D), resulting in an ion cloud that enters the TOF mass analyzer. The ions are separated based on their mass and are accelerated to the detector (E). The detector measures the quantity of each isotope for each individual cell in the sample; data is generated in an FCS format (G) and analyzed (H).

* Picture is taken from Fluidigm


In special circumstances, we develop and support an entire mass cytometry based experimental workflow. We stand at your disposal for discussion using the mass cytometry technology in your work.

For Additional Information:

Carsten Krieg, Ph.D.