Understanding the complexities of redox mediated signaling events requires a multidisciplinary approach. The SC COBRE in Oxidants, Redox Balance and Stress Signalling has assembled a cohort of promising junior faculty with expertise in relevant biomedical model systems. Analytical biochemistry specific to the detection and quantification of redox sensitive molecules and coordinate protein changes that drive homeostasis is a unique niche fulfilled by the Analytical Redox Biology Core (ARBC).
Cellular redox species are produced directly or indirectly via bioenergetic metabolic reactions. Although the leak of electrons is often cited as the primary source of superoxide and hydroxy radicals, the leak is only a small contributor to the many different species involved in cellular redox reactions. Indeed, the fundamental basis of bioenergetics involves the oxidation (loss of electrons) of reduced nutrients and subsequent production of metabolites with a range of redox potentials (i.e., nicotinamides, reduced/oxidized metalloproteins, and thiol-containing species with varied redox potentials. Profiling the flux of these various metabolites through their attendant metabolic reactions is fundamental to any studies aimed at understanding cellular redox reactions.
Cell and Molecular Imaging (CMI) Core D provides COBRE investigators access and assistance for high end laser scanning confocal/multiphoton/super-resolution microscopy and related imaging techniques. Core D houses five confocal/multiphoton systems: 1) a state-of-the-art Zeiss LSM 880 NLO Quasar confocal/multiphoton microscope with a Fast Airyscan super-resolution detector; 2) an Olympus FV1200 silicone oil optics multiphoton microscope configured especially for intravital imaging; 3) an Olympus FV10i LIV confocal microscope with water immersion optics for live cell imaging; 4) a Zeiss LSM 510 META laser scanning confocal microscope for general purpose imaging of live and fixed specimens; and 5) a BD CARV II disk-scanning confocal microscope for video rate “real-time” confocal imaging.
The goal of the SC COBRE in Oxidants, Redox Balance and Signaling Proteomics Core is to provide state-of-the-art LC-MS/MS instrumentation, expertise, and training for comprehensive proteomic analyses to advance the research endeavors and career development of junior investigators with interests in redox signaling. Dedicated core personnel assist with experimental design, method development, data acquisition, and computational analysis for protein characterization and quantitative proteomic applications using metabolic labeling (SILAC), isobaric tandem mass tagging (TMT), and label free quantitation (MaxQuant LFQ).